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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9

Fig. 5

miR-690 directly targeted Sox9 in iPSCs-derived IPCs. a Predicted miR-690 targeting sequence in the 3′UTR of Sox9 (Sox9 WT-3′UTR) and the mutant form of the Sox9 3′UTR (Sox9 MUT-3′UTR). b Dual-luciferase reporter assays to determine the influence of miR-690 on Sox9 3′UTR activity in iPSCs-derived IPCs. Data are the mean ± SD of three independent assays. ce Quantitative RT-PCR and Western blotting analyses of the effects of miR-690 knockdown (miR-690 inhibitor) on the expression level of Sox9 and the effects of miR-690 knockdown (miR-690) on the repressive effects of Sox9 knockdown (Sox9 siRNA). β-Actin was used as the loading control. GAPDH was used as the internal control for mRNA. Error bars show the SD (n = 3)

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