Fig. 3

Analysis of markers of endometrium, inflammation and MenSCs secretion in uterus of four treatment groups. a Representative immunohistochemical staining of CK-18 in uterus at day 9 and day 18 (200×). b Representative immunohistochemical staining of Vimentin in uterus at day 9 and 18 (200×). c Immunofluorescent staining of GFP (green) and PDGFRβ (red) in uterus of M group and MP group at day 9 and day18. Nucleus was stained with DAPI (blue), while yellow staining indicated the co-expression of GFP and PDGFRβ (200×). d Comparison of CK-18 and Vimentin intensity at day 9 and day 18 (N≥6). e Real-time PCR analysis of rat genes including IL-1β, IL-4, IL-6and IL-10in uterus (N≥6). f LUMINEX analysis of rat IL-1β, IL-4, IL-6 and IL-10 in uterus(N≥6). g Real-time PCR analysis of human genes includingIGF-1,SDF-1and TSP-1between M group and MP group at day 18, data was normalized to human GAPDH (N=8)