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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The role of the vasculature niche on insulin-producing cells generated by transdifferentiation of adult human liver cells

Fig. 3

ECFC and MSC co-culturing in the Transwell® system promotes pTF induced liver-to-pancreas transdifferentiation in vitro. Induced IPCs were cultured on the bottom of the Transwell®; inserts were plated with ECFCs or MSCs or ECFCs + MSCs (1:1 ratio) or with IPCs as a control, as illustrated in a. After 3 days, the transdifferentiation efficiency of the cells was measured, as seen in b: the transcript levels of the pancreatic-specific genes are presented as average and SE for increases in the above cells treated with pTFs only, cultured in a regular 12 wells/plate (TC plate) and normalized to β-ACTIN levels, *P value < 0.05 and **P value < 0.01, respectively. c Glucose-regulated insulin secretion was measured using a specific human RIA kit (DPC). The secretion in the cells that were cultured in the Transwell® system was compared to cells cultured in a regular 12 wells/plate (TC plate). Results are presented as average and SE, n ≥ 12 from four independent repeats in different donors, *P value < 0.01 compared to cells treated with pTFs only, cultured in a regular TC plate treatment. The labeling below the graphs indicates the cell type cultured in the inserts of the Transwell® system

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