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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway

Fig. 2

Continuous single-dose ISO time-dependently delayed C2C12 cells differentiation and myoblast fusion. a The typical image of myoblast fusion day 2, day 4 and day 6 after C2C12 cells differentiation with or without continuous single-dose ISO stimulation as determined by immunofluorescent staining of MyHC. Green color indicates MyHC; blue color indicates DAPI for nuclear labeling. b Continuous single-dose ISO prominently depressed the numbers of MyHC-positive cells day 2 after C2C12 cells differentiation. *P = 0.0000 vs. Ctrl. c Continuous single-dose ISO remarkably decreased the myotube numbers of more than 5 myoblast fusion day 4 after C2C12 cells differentiation. *P = 0.0000 vs. Ctrl. d Continuous single-dose ISO markedly reduced the myotube numbers of more than 5 myoblast fusion day 6 after C2C12 cells differentiation. *P = 0.0000 vs. Ctrl

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