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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway

Fig. 5

Continuous single-dose ISO delayed C2C12 cells differentiation and myoblast fusion through altering ratio of PKA RI/RII. a The expression of indicated proteins in continuous single-dose ISO-treated C2C12 cells were assessed by Western blot. α-tubulin as the internal control. b-e Continuous single-dose ISO obviously reduced levels of PKA RIα and PKA RIβ in C2C12 cells while increasing the levels of PKA RIIα as semi-quantitative analyses of PKA different subtypes band intensities. PKA RI/RII expressed the ratio of PKA RIα+PKA RIβ to PKA RIIα band intensities. #P < 0.001 vs. another groups; &P = 0.0014 vs. 10-8 M ISO; $P = 0.0005 vs. 10-7 M ISO; *P = 0.0019 vs. 10-6 M ISO; n = 3. f The typical image of myoblast fusion of continuous single-dose ISO-treated C2C12 cells differentiation with or without PKA RI activator N6-Bz-cAMP (10-4 M) or PKA inhibitor H-89 (10-7 M) as determined by immunofluorescent staining of MyHC. Green color indicates MyHC; blue color indicates DAPI for nuclear labeling. g PKA RI activator partially abolished the inhibitory effects of continuous single-dose ISO on C2C12 cells differentiation while PKA inhibitor worsened its roles. &P = 0.0004 vs. Ctrl; $P = 0.0007 vs. 10-5 M ISO; *P < 0.05 vs. 10-5 M ISO; #P = 0.0016 vs. N6-Bz-cAMP; n = 3

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