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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway

Fig. 7

ERK1/2-mediated inactivation of β2-AdR involved in C2C12 cells differentiation and myoblast fusion. a-b Continuous single-dose ISO decreased MyHC expressions, the specific effect could be partially abolished by PD98059 as determined by western blot. &P = 0.0016 vs. Ctrl; *P = 0.0011 vs. 10-5 M ISO; n = 3. c The typical image of myoblast fusion as determined by immunofluorescent staining of MyHC. Green color indicates MyHC; blue color indicates DAPI for nuclear labeling. d-f PD98059 obviously abrogated the effects of continuous single-dose ISO on the numbers of MyHC positive myotube derived from C2C12 cells differentiation. &P = 0.002 vs. Ctrl; $P < 0.05 vs. 10-5 M ISO; *P = 0.0014 vs. 10-5 M ISO; @P = 0.041 vs. 10-5 M ISO; n = 6. g-i PD98059 partially abolished the effects of ISO on myotube numbers with different length derived from C2C12 cells differentiation. &P = 0.0000 vs. Ctrl; @P = 0.0025 vs. Ctrl; *P = 0.009 vs. 10-5 M ISO; $P < 0.05 vs. 10-5 M ISO. n = 6

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