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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Continuous exposure of isoprenaline inhibits myoblast differentiation and fusion through PKA/ERK1/2-FOXO1 signaling pathway

Fig. 8

ERK1/2 involved in C2C12 cells differentiation and muscle fiber types through FoxO1 signaling. a-d ERK1/2 inhibitor PD98059 partially abrogated the role of continuous single-dose ISO in repressing the expression of MyHC1 and MyHC2a as detected by Real-time PCR, in addition to further reducing the mRNA levels of MyHC2b and MyHC2x. &P = 0.0000 vs. Ctrl; *P = 0.0292 vs. 10-5 M ISO; #P = 0.0004 vs. Ctrl; n = 6. e-g PD98059 in part recovered the decreased levels of pAKT, and p-p38MAPK in ISO-treated C2C12 cells as determined by Western blot and band intensities. α-tubulin as the internal control. &P = 0.0004 vs. Ctrl; *P = 0.0122 vs. 10-5 M ISO; n = 3. h-i ERK1/2 inhibitor PD98059 partially decreased the levels of nuclear FOXO1 in continuous single-dose ISO-treated C2C12 cells as analyzed by Western blot band intensities. &P = 0.0004 vs. Ctrl; #P = 0.0124 vs. 10-5 M ISO; n = 3. j ERK1/2 inhibitor PD98059 partially reduced nuclear translocation of FOXO1 in differentiated C2C12 cells induced by ISO as detected by immunofluorescent staining. Red: FOXO1; green: MyHC; blue: DAPI-labeled nuclei

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