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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Long noncoding RNA LINC00707 sponges miR-370-3p to promote osteogenesis of human bone marrow-derived mesenchymal stem cells through upregulating WNT2B

Fig. 5

LINC00707 negatively regulate miR-370-3p during osteogenic induction a mRNA levels of RUNX2, ALP, and OCN detected by qRT-PCR in HBMSCs co-transfected with LINC00707/NC and miR-370-3p mimics/mimics NC after osteogenic induction for 7 days. b mRNA levels of RUNX2, ALP, and OCN were detected by RT-PCR in HBMSCs co-transfected with sh-LINC00707/sh-NC and miR-370-3p inhibitor/inhibitor NC after osteogenic induction for 7 days. c Protein level of RUNX2 in HBMSCs co-transfected with LINC00707/NC and miR-370-3p mimics/mimics NC after osteogenic induction for 7 days. d Protein level of RUNX2 in HBMSCs co-transfected with sh-LINC00707/sh-NC and miR-370-3p inhibitor/inhibitor NC after osteogenic induction for 7 days. e, g Relative activity of ALP, ARS staining, and ALP staining in HBMSCs co-transfected with LINC00707/NC and miR-370-3p mimics/mimics NC after osteogenic induction for 7 days. f, h Relative activity of ALP, ARS staining, and ALP staining in HBMSCs co-transfected with sh-LINC00707/sh-NC and miR-370-3p inhibitor/inhibitor NC after osteogenic induction for 7 days. Data represent the mean ± S.D. *P<0.05, **P<0.01

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