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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Long noncoding RNA LINC00707 sponges miR-370-3p to promote osteogenesis of human bone marrow-derived mesenchymal stem cells through upregulating WNT2B

Fig. 6

WNT2B is a LINC00707/miR370-3p axis target during osteogenesis. a Schematic diagram of miR-370-3p putative binding sites in wild type and mutant WNT2B 3′-UTR. b Binding sites of miR-370-3p to wild type or mutant WNT2B 3′UTR under transfection with miR-370-3p mimics were determined with dual-luciferase reporter assay. c WNT2B protein level determined in HBMSCs transfected with miR-370-3p mimics or inhibitor using Western blotting. d WNT2B mRNA level determined in HBMSCs transfected with miR-370-3p mimics or inhibitor using qRT-PCR. e WNT2B mRNA level was determined in HBMSCs at 0, 5, 10, 15, and 20 days after osteogenic induction by qRT-PCR. f WNT2B mRNA level was determined in LINC00707-overexpressing or LINC00707-silenced HBMSCs. g Correlation analysis was performed between miR-370-3p and LINC00707 levels at indicated times after osteogenic induction. h Correlation analysis was performed between miR-370-3p and LINC00707 RPKM values tested by high-throughput sequencing analysis after osteogenic induction. i WNT2B expression levels detected by Western blot assay in HBMSCs, co-transfected with LINC00707/NC and miR-370-3p mimics/mimics-NC after osteogenic induction for 7 days. j, k RIP assay of enrichment of Ago2 on LINC00707 and WNT2B transcripts relative to IgG in HBMSCs transfected with LINC00707-overexpressing or LINC00707-silenced lentivirus and their corresponding controls. Data represent the mean ± S.D. *P<0.05, **P<0.01

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