Fig. 1

Strategy for generation, expansion, and characterization of human embryonic stem cell-derived neural stem cells. a Schematic diagram depicting the experimental design of in vitro ES-NSCs generation and in vitro and in vivo post-grafting characterization. b, c Morphology of NSCs derived by FACS Sorting (FACS-NSCs) and clonal morphology manual selection (CoMo-NSCs) at passage 10 and 15. d, e Expression of NSC-specific and ESC-specific markers determined by flow cytometry at passages less than 15 (d) and greater than 15 (e). Data are represented as mean ± SEM. f Differential gene expression plot showing the log-fold change and average transcripts per million (TPM) of each gene when comparing CoMo-NSCs and FACS-NSCs. Gray dots represent genes that are not significantly different between the two groups; the absence of black dots seen in the plot indicate that there were no genes that were significantly different between the two groups. g Heat map of log2(TPM+1) values of genes that distinguish ESCs from FACS-NSCs across ESC, FACS-NSC, and CoMo-NSC samples. The selection of genes is described in the methods (scale bars: b, c 50 μm)