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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors

Fig. 2

In vitro proliferating clonal morphology-derived NSCs (CoMo-NSCs) show consistent morphology and expression of markers characteristic of immature NSCs. a, b Characteristic stable morphology of CoMo-NSCs in low (a) and high (b) density. c Expression of selected NSC markers (Sox1, Sox2, Nestin, CD24, Pax6, and CD44) evaluated by flow cytometry at different passages. Data are represented as mean ± SEM. d–f Expression of NSC-specific markers (Sox2, Nestin, Plzf, Dach-1, N-cadherin) and tight junction protein ZO-1 in undifferentiated CoMo-NSCs as determined by indirect immunofluorescence (scale bars: a, b 25 μm; d–f 10 μm)

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Stem Cell Research & Therapy

ISSN: 1757-6512