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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Inhibition of PTGS1 promotes osteogenic differentiation of adipose-derived stem cells by suppressing NF-kB signaling

Fig. 2

Knockdown of PTGS1 enhances the osteogenic differentiation of ASCs in vitro. a GFP-positive ASCs under light and fluorescence microscopy. Scale bar, 500 μm. b, c Knockdown of PTGS1 was verified by real-time RT-qPCR and Western blot. d, e PTGS1 silencing increased the ALP activity and ALP staining in ASCs. f, g PTGS1 knockdown increased mineralization, as shown by Alizarin red staining and quantitative calcium analysis. hl The mRNA expressions of ALP, OCN, BSP, RUNX2, and OSX were determined by RT-qPCR. GAPDH was used as an internal control. All data are shown as the mean ± SD, n = 3. **P < 0.01. NC negative control cells, PTGS1sh PTGS1 knockdown cells, d day, w week

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