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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Inhibition of PTGS1 promotes osteogenic differentiation of adipose-derived stem cells by suppressing NF-kB signaling

Fig. 5

Knockdown of PTGS1 inhibits the translocation of p65 from the cytoplasm to the nucleus. ac Real-time RT-qPCR showed that expressions of p65 target genes IL6, IL8, and SELE were reduced when PTGS1 was silenced with a short hairpin RNA. GAPDH was used as an internal control. d The protein levels of p-IκBα, total IκBα, p-p65, and total p65 were measured by Western blot in the absence and presence of TNFα for 0.5 h. e The cellular localization of endogenous p65 was observed by confocal microscopy in both control and PTGS1 knockdown cells with or without TNFα treatment. Scale bars, 100 μm. f The nuclear and cytoplasmic levels of p65, tubulin, and PCAF were measured by Western blot after subcellular fractionation from PTGS1sh cells untreated or treated with TNFα for 0.5 h. GAPDH was used as an internal control. All data are shown as the mean ± SD, n = 3. **P < 0.01. NC negative control cells, PTGS1sh PTGS1 knockdown cells

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