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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: miRNA-21 promotes osteogenesis via the PTEN/PI3K/Akt/HIF-1α pathway and enhances bone regeneration in critical size defects

Fig. 2

miRNA-21 promotes osteogenesis by upregulating P-Akt expression levels. a HIF-1α, VEGF, BMP-2, OPN, OCN, and Runx2 protein levels were detected via western blotting after BMSCs were infected with Lenti-LacZ or Lenti-miRNA-21 at the indicated time points. GAPDH served as the loading control. b UCBMSCs were transfected with 0 μg, 0.2 μg, 0.4 μg, 0.8 μg, 1.6 μg [4], or 3.2 μg of miR-21 mimics at 0 day and 7 days, and cells then were collected at 14 days. HIF-1α, VEGF, BMP-2, OPN, OCN, and Runx2 protein levels were detected via western blotting. GAPDH served as the loading control. c PTEN, PI3K, Akt, and HIF-1α protein levels were detected via western blotting at 48 h after BMSCs were transfected with control or miRNA-21 mimics. GAPDH served as the loading control. d Histogram showing the relative miRNA-21 levels detected by qRT-PCR after BMSCs were transfected with a control or miRNA-21 mimics. e PTEN, PI3K, Akt, and HIF-1α protein levels were detected via western blotting at 48 h after BMSCs were transfected with control or miRNA-21 inhibitor. GAPDH served as the loading control. f Histogram showing the relative miRNA-21 levels detected by qRT-PCR after BMSCs were transfected with a control or miRNA-21 inhibitor. g PTEN, P-Akt, Akt, and HIF-1α protein levels were detected via western blotting after BMSCs were transfected with control or miRNA-21 mimics or exposed to LY294002. GAPDH served as the loading control. h Histogram showing the relative miRNA-21 levels detected by qRT-PCR after BMSCs were transfected with control or miRNA-21 mimics or treated with LY294002

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