Fig. 3

The effect of overexpressing p130 or E2F4 on the retention of mMSCs in the lungs after LPS challenge (immunofluorescent imaging). a Representative immunofluorescent staining images of the lung tissues from mice at 7 and 14 days after mMSC administration. mMSC engraftment in the lung tissues is shown as GFP-positive cells (green). Nuclei of cells were stained with DAPI (blue). Double-stained cells with both GFP and DAPI were light green. Representative photographs are shown from three mouse lungs at 7 and 14 days after mMSC administration. All microphotographs were acquired at × 200 magnification (scale bar = 50 μm). b Fluorescent counts in injured lungs from mice at 7 and 14 days after mMSC administration. Overlapping fluorescence of DAPI and GFP was counted in randomly selected fields (count/field) (n = 3; *p < 0.05 compared with the LPS+MSC-NC group). mMSCs mouse mesenchymal stem cells, GFP green fluorescent protein