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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model

Fig. 1

Schematic representation of ischemia/reperfusion injury experimental setup. Ischemia was mimicked by replacing expansion medium by ischemic mimetic solution (IMS) and by culturing cells in a N2 gaseous environment. After 5 h of ischemia, reperfusion was mimicked by re-establishing normoxic culture conditions (ExpM at 3% O2). I/R setup was performed using mono-cultures of hiPSC-CMs, mono-cultures of hCPCs, and co-cultures of the two cell types using transwell permeable inserts. The impact of the I/R was evaluated regarding hCPC proliferation, hiPSC-CM viability, hCPC whole proteome, and secretion of IGF-1 and CXCL6 factors in different time points: (BI—before injury; Post I—post ischemia; Post R—post reperfusion). Control cultures were done in parallel (using normoxic conditions)

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