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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model

Fig. 5

Angiogenic functional evaluation of I/R conditioned medium. Ischemic mimetic solution (IMS), endothelial cell growth medium 2 (ECGM2), and conditioned media of hCPC mono-culture post ischemia (M CPC i Post I) and of co-culture post ischemia (Co i Post I) conditions were tested for angiogenic potential by HUVECS tube formation assay. a Total segment length, total branching length, and number of nodes are represented. ¤¤ p < 0.01; ¤¤¤ p < 0.005; ¤¤¤¤ p < 0.0001 vs IMS. b Representative original images (left) and quantification by angiogenesis analyzer are shown. In the right image, there is an indication of master junctions (pink dots), master segments (yellow), meshes (light blue), branches (green), and isolated segments (blue). Scale bars 200 μm

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