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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mesenchymal stem cell-loaded porous tantalum integrated with biomimetic 3D collagen-based scaffold to repair large osteochondral defects in goats

Fig. 1

Identification of BMSCs and chondrocytes. BMSCs were identified with the surface markers a CD79a− (1.26%), b HLA-II− (1.45%), d CD29+ (97.02%), e CD14− (0.58%), g CD44+ (96.34%). c, f, h The negative control was generated by replacing the antibody with isotype IgG. BMSCs in passage 3 cultured in osteogenic induction medium for (i) alkaline phosphatase were detected in the cytoplasm after induction of osteogenic differentiation for 14 days. j Alizarin red staining showed small calcium nodules in unlabeled after induction of osteogenic differentiation for 21 days. k Adipogenesis was examined using oil red O staining. l Alison blue staining was performed to assess the chondrogenic capacity. m Light microscopy of chondrocytes. n Toluidine blue staining of chondrocytes. o Immunocytochemistry of collagen II with chondrocytes. All black bar = 200μm

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Stem Cell Research & Therapy

ISSN: 1757-6512