Table 2 Sequences of oligonucleotide primers used to amplify the listed mRNA species, and PCR product sizes
Target gene | Primer sequence | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
ALP | F:tacttgtgcggggtgaagg | 60 | 144 |
R:cacgatgcccacggattt | |||
OSX | F:ggcaaagcaggcacaaaga | 60 | 184 |
R:ctgggaacgagtgggaaaag | |||
OCN | F:cagcgaggtggtgaagaga | 60 | 144 |
R:tggaagccgatgtggtcag | |||
Col-I | F:tggtgaagcaggcaaacct | 60 | 87 |
R:aaacctctctcgcctcttgct | |||
OSN | F:tcgactcttcctgccacttct | 60 | 200 |
R:gttgtcctcgtccctctcgt | |||
RUNX2 | F:ccgaaatgcctctgctgtt | 60 | 87 |
R:aaactcttgcctcttccactcc | |||
Col-II | F:ctcaagtccctcaacaaccaga | 60 | 123 |
R:ccagtagtctccgctcttcca | |||
Agg | F:cctcaccatcccctgctactt | 60 | 119 |
R:gcaccacctccttctccttg | |||
SOX9 | F:caagttccccgtctgcatc | 60 | 111 |
R:tgcggcttgttcttgctc | |||
Col-X | F:gaacggcacccctgtaatgt | 60 | 99 |
R:tggtcgttctcggtgaggt | |||
GAPDH | F:ttgtgatgggcgtgaacc | 60 | 127 |
R:ccctccacgatgccaaa |