Fig. 3From: Differentiation roadmap of embryonic Sertoli cells derived from mouse embryonic stem cellsObserving the induction process with FCM, IF, ICC, and qPCR. Optical micrographs a and b show the morphological characteristics of the PCs of mES+MEF and mES+Trans groups. In a, scale bar = 200 μm and 400 μm for the left and right panels, respectively. In b, scale bar = 200 μm. c Optical micrographs and IF were performed in group mES+Trans at day 20. Brightfield and fluorescence of AMH (green) images were merged on the left. Fluorescence of FasL (green) and Dapi (blue) merged imaging on the right. Scale bar = 200 μm. d ICC was performed with FasL antibody in groups mES+MEF and mES+Trans at day 30. Dark brown color shows the positive cells. Pale brown and achromatic colors show the negative cells. Scale bar = 400 μm. e At day 35 in group mES+Trans, tubular-like structure colonies were observed. Results show in optical micrographs on the left, and fluorescence merging images FasL (green) and Dapi (blue) on the right. Scale bar = 200 μm. f At day 30, FasL-positive cells formed ring-like structures in group mES+Trans. Scale bar = 100 μmBack to article page