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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction

Fig. 1

Morphology changes in adipose-derived mesenchymal stem cells (ADMSCs) in vitro treated by different methods on days 3, 7, 14, and 21. a–d ADMSCs treated with DMEM/F12 medium containing 10% FBS (GM). e–h ADMSCs treated with retinoic acid (RA) and testosterone (T) (DM). Cells stopped proliferation and formed loose cell clones on induced 14 days and 21 days (black arrow). i–l ADMSCs were indirectly co-cultured with TM4 cell in combination with RA and T treatment (IC). Cells stopped proliferation and formed loose cell clones on induced 7 days, tightly packed and flat clones were observed on induced 21 days (black arrow). m–p ADMSCs directly co-cultured with TM4 cell in combination with RA and T treatment (DC). Cells stopped proliferation and formed loose cell clones on induced 7 days; the clones are tightly packed up to 14 days (black arrow). r Morphology of mitomycin C inactivated TM4 cells on day 21

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Stem Cell Research & Therapy

ISSN: 1757-6512