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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction

Fig. 4

Male germ cell-related markers expression in ADMSCs after treating with RA, T, TM4 cell, and TM4 cell-conditioned medium (TCCM) for 21 days by qRT-PCR, WB and flow cytometry (FCM) analysis. DM: treatment with RA and T (white bar); IC: combination of RA and T treatment and indirect co-culture with TM4 cell (black bar); TM4 cell: indirect co-culture with mitomycin C inactivated TM4 cell (low gray bar); TCCM: treatment with TCCM (gray bar); TCCM+RA+T: treatment with TCCM, RA and T (dark gray bar). a Relative mRNA expression in ADMSCs by qRT-PCR analysis. Y-axis indicates the relative mRNA value normalized to ADMSCs from DM group (white bar). b Relative protein expression in ADMSCs by WB analysis. Y-axis indicates the relative gray value normalized to ADMSCs from DM group (white bar). c DDX4 expression in ADMSCs by FCM analysis. Bar graphs represent mean ± SD (n = 3 per group). Different letters on the bar graph indicate significant differences between the groups at the 0.05 level (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512