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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction

Fig. 9

Expression of male GC-related markers in 5 and 12.5 μM ruxolitinib-treated ADMSCs and 0.25 and 0.5 μM niclosamide-treated ADMSCs and untreated cells on 21d. a, b The mRNA expression of GC-related markers Stella, DDX4, Dazl, PGP9.5, and ITGα6 in ADMSCs were evaluated by qRT-PCR. ADMSCs were treated with ruxolitinib (RUX) (a, c, e). ADMSCs were treated with niclosamide (NICLO) (b, d, f). a, b The mRNA expression of GC-related markers Stella, DDX4, Dazl, PGP9.5, and ITGα6 in ADMSCs were evaluated by qRT-PCR. c The protein expression of phosphorylation of JAK2 (p-JAK2) and total JAK2 in ADMSCs was examined by western blot. d The protein expression of phosphorylation of STAT3 (p-STAT3) and total STAT3 in ADMSCs was examined by western blot. e, f The protein expressions of GC-related markers in ADMSCs were examined by western blot. Different letters on the bar graph indicate significant differences between the groups at 0.05 level (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512