Fig. 3

hUC-MSC infusion blocks ALF development by inhibiting the activation of circulating monocytes. a ELISA measurement of serum levels of sCD163 in the treated monkeys. N = 6. b Representative photomicrographs of the results of immunohistochemical staining to identify resident KCs (CD68+) and recruited hepatic macrophages (MAC387+) in liver tissues. c Quantitation of CD68+ and MAC387+ macrophages. The numbers of CD68+ and MAC387+ cells were counted in 10 consecutive high-power fields in three monkeys. For each section, 10 randomly chosen portal tracts or areas of central veins were assessed at high magnification (× 400), and the cumulative number of positive cells in 10 HPF was recorded. d Transcript levels of IL-6 in liver homogenates, circulating monocytes, and lymphocytes. Each bar represents data obtained from at least three independent quantitative PCR experiments. e, f Flow cytometry analysis of activated monocytes (CD14+CD16+CCR2+) in subsets of peripheral blood monocyte, N = 6. Error bars, SEM. Student’s t test, *P < 0.05, ^&P < 0.01, and ^$P < 0.001