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Table 3 Induced pluripotent stem cells in wound healing

From: The potential and limitations of induced pluripotent stem cells to achieve wound healing

Author Year Cell type Delivery method Cell number Animal model Major findings
Clayton et al. [42] 2018 hiPSC-derived endothelial cells Intradermal injection
Suspended in medium and Matrigel
5 × 105 Nude mice
Non-diabetic
1. Increased angiogenesis
2. Accelerated wound closure
3. Increased collagen deposition, macrophage number, blood vessel density
4. Increased host expression of PECAM, Tie-1, and VEGF
5. Increased wound perfusion
Kim et al. [43] 2013 hiPSC-derived endothelial and smooth muscle cells Intradermal injection in PBS 6 × 104 EC + 4 × 104 SMC Nude mice
Non-diabetic
1. Increased angiogenesis
2. Accelerated wound closure
3. Increased in vitro VEGF, EGF, and FGF-4
4. Increased smooth muscle cell migration
Shen et al. [48] 2016 hiPSC early vascular cells Topical application
Acrylated hyaluronic acid hydrogels
Variable Nude mice
STZ diabetic
1. Accelerated wound closure and perfusion
2. No significant difference between healthy and diabetic donor derived cells
3. Increased blood vessel density
Tan et al. [49] 2018 hiPSC-derived endothelial cells Topical application
Electrospun PCL/gelatin scaffolds
1 × 105 FVB/N mice
Non-diabetic
1. Increased angiogenesis compared to controls
2. Increased cell survival in scaffolds compared to cellular injections
3. Increased arteriole density in scaffold group compared to control and cellular injections
Kashpur et al. [55] 2018 hiPSC-derived fibroblasts Topical application
Polyethylene terephthalate membrane self-assembled tissues
16,000 Nude mice
STZ diabetic
1. Accelerated wound healing with hiPSC-derived fibroblasts from DFU compared to primary cells
2. No difference in gene expression between hiPSC-derived fibroblasts derived from healthy and diabetic patients
Nakayama et al. [57] 2018 hiPSC-MSC Intravenous injection 1 × 106 and 3 × 105 Nude mice
Non-diabetic
1. Accelerated wound healing as measured by epithelialization after IV delivery of 1 × 106 cells
Zhang et al. [59] 2015 hiPSC-MSC-derived extracellular vesicles Intradermal injection + topical application in PBS 200 μg SD rats
Non-diabetic
1. Increased angiogenesis
2. Accelerated wound healing
3. Increased collagen density
4. Increased blood vessel density
Kobayashi et al. [60] 2018 hiPSC-derived extracellular vesicles Intradermal injection + topical application in PBS 20 μg C57 mice
db/db diabetic
1. Increased angiogenesis
2. Accelerated wound healing
3. Increased in vitro fibroblasts migration and replication
  1. Summary of studies implying induced pluripotent stem cells in cutaneous wound healing in a murine model, including cell type, delivery method, animal model, and major findings
  2. STZ streptozocin, SD Sprague-Dawley