From: The potential and limitations of induced pluripotent stem cells to achieve wound healing
Author | Year | Cell type | Delivery method | Cell number | Animal model | Major findings |
---|---|---|---|---|---|---|
Clayton et al. [42] | 2018 | hiPSC-derived endothelial cells | Intradermal injection Suspended in medium and Matrigel | 5 × 105 | Nude mice Non-diabetic | 1. Increased angiogenesis 2. Accelerated wound closure 3. Increased collagen deposition, macrophage number, blood vessel density 4. Increased host expression of PECAM, Tie-1, and VEGF 5. Increased wound perfusion |
Kim et al. [43] | 2013 | hiPSC-derived endothelial and smooth muscle cells | Intradermal injection in PBS | 6 × 104 EC + 4 × 104 SMC | Nude mice Non-diabetic | 1. Increased angiogenesis 2. Accelerated wound closure 3. Increased in vitro VEGF, EGF, and FGF-4 4. Increased smooth muscle cell migration |
Shen et al. [48] | 2016 | hiPSC early vascular cells | Topical application Acrylated hyaluronic acid hydrogels | Variable | Nude mice STZ diabetic | 1. Accelerated wound closure and perfusion 2. No significant difference between healthy and diabetic donor derived cells 3. Increased blood vessel density |
Tan et al. [49] | 2018 | hiPSC-derived endothelial cells | Topical application Electrospun PCL/gelatin scaffolds | 1 × 105 | FVB/N mice Non-diabetic | 1. Increased angiogenesis compared to controls 2. Increased cell survival in scaffolds compared to cellular injections 3. Increased arteriole density in scaffold group compared to control and cellular injections |
Kashpur et al. [55] | 2018 | hiPSC-derived fibroblasts | Topical application Polyethylene terephthalate membrane self-assembled tissues | 16,000 | Nude mice STZ diabetic | 1. Accelerated wound healing with hiPSC-derived fibroblasts from DFU compared to primary cells 2. No difference in gene expression between hiPSC-derived fibroblasts derived from healthy and diabetic patients |
Nakayama et al. [57] | 2018 | hiPSC-MSC | Intravenous injection | 1 × 106 and 3 × 105 | Nude mice Non-diabetic | 1. Accelerated wound healing as measured by epithelialization after IV delivery of 1 × 106 cells |
Zhang et al. [59] | 2015 | hiPSC-MSC-derived extracellular vesicles | Intradermal injection + topical application in PBS | 200 μg | SD rats Non-diabetic | 1. Increased angiogenesis 2. Accelerated wound healing 3. Increased collagen density 4. Increased blood vessel density |
Kobayashi et al. [60] | 2018 | hiPSC-derived extracellular vesicles | Intradermal injection + topical application in PBS | 20 μg | C57 mice db/db diabetic | 1. Increased angiogenesis 2. Accelerated wound healing 3. Increased in vitro fibroblasts migration and replication |