Fig. 2

C-MYC overexpression in MSC correlated with its functional activity. Bone marrow-derived MSC transduced with either c-MYC or GFP (control) were expanded and collected at passages 2–4 (early passages) or 9–10 (late passages). a, d, e mRNA expression of indicated genes was measured by qRT-PCR analysis in indicated MSC at early or late passages, as indicated, n = 4; *P < 0.05, versus GFP-MSC at similar passages. b Western blot analysis of c-MYC protein abundancies in indicated MSC at early or late passages. β-Actin served as a loading control. c c-MYC DNA-binding activity was measured by ELISA assay in indicated MSC at passage 10 (n = 3) and quantified as the amount of c-MYC (μg/ml) bound to an oligonucleotide containing a c-MYC consensus sequence; *P < 0.05 versus GFP control