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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells

Fig. 6

C-MYC overexpression did not prevent ectopic bone formation by human MSC in vivo. Bone marrow-derived MSC from five donors were transduced either with c-MYC or GFP, or were left non-transduced (control MSC), then expanded to passage 2 and used for β-TCP/MSC construct preparations which were implanted into SCID mice (n = 8) for ectopic bone formation during 6 weeks. a Representative images of HE staining of β-TCP explants with corresponding MSC, as indicated; scale bar, 250 μm; b histomorphometrical analysis of 48–54 sections for each group; *P < 0.05, versus control non-transduced MSC. c In situ hybridization analysis of explanted β-TCP/MSC constructs after ectopic bone formation done in five to six sections for each group, either with a probe specific to human genomic ALU sequences or to murine genome repetitive elements Sine, as indicated; right: HE staining of corresponding sections indicating ectopic bone positions; black arrows indicate positions of nuclei with positive staining for murine hybridization probe; scale bar, 20 μm. B—ectopic bone tissue; F—fibrous tissue; β-TCP—β-TCP debris

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