Fig. 4
The administration of hADSCs increases the angiogenesis in mouse model of the hindlimb ischemia. Capillaries (CD31 positives cells) count in collected muscles 7, 14, and 21 days after the artery ligation and the administration of hADSC or PBS− (a). The Number of capillaries in collected muscles 14 days after the artery ligation and the administration of hADSC, mADSC, mBM-MSC, L929, GM07492, NHDF, and PBS− (b). The number of the vessels (CD31+) in each group was determined in 10 fields of view at a magnification of 40x and calculated per 1mm2 of tissue (n = 5; 5 muscles per group were analyzed; 5 (for total area of F4/80) or 10 (for number of capillaries) pictures of each muscle were taken). **p < 0.05, ***p < 0.001 evaluated with Kruskal-Wallis one-way analysis of variance and multiple comparison of mean ranks for all groups (hADSCs statistically significant with each group except the mADSC group, p = 0.235). Nuclei stained with DAPI (blue), scale bars = 50 μm. The immunostaining of pericytes CD146 (red)/endothelial cells (green) with nuclei staining (blue) (c). The immunostaining of αSMA (green)/endothelial cells (red) with nuclei staining (blue) (d)