Fig. 5

hADSCs secrete interleukin 6. The levels of IL-6 secreted by hADSCs (a) and mADSCs (b) in vitro were determined by ELISA. The amounts of secreted IL-6 were calculated for 1 mg of total protein content. The immunofluorescence staining of interleukin 6 (green) and hADSCs (red) (c). The representative images of the double immunofluorescence staining of human specific Lamin A/C (green) and interleukin 6 (red) (d, e). The images show that the injected hADSC localized in clusters, presumably at the injection site, and interleukin 6 was located around hADSCs on the third (d) and the seventh (e) day after the cell injection in the ischemic limb muscles. The amounts of the secreted IL-6 by human ADSCs (f) and murine ADSCs (g) in the murine muscle and serum was determined by ELISA test on the 3rd after artery ligation (n = 5). **p < 0.01 evaluated with U Mann-Whitney test. Nuclei were stained with DAPI (blue), scale bars = 50 μm