Fig. 7

The administration of the hADSCs into the ischemic muscle of immunodeficient NOD SCID mice increases the number of new blood vessels but does not increase the macrophages infiltration. The representative images of H&E staining of the transverse sections of gastrocnemius muscle after PBS⁻ (a, b) and hADSCs (c, d) injection on days 7 and 14 post surgery. Necrotic muscle fibers (black arrows) with pale cytoplasm and regenerative small, basophilic muscle fibers with one or more centrally located nuclei (white arrows) were observed at 7 days after PBS⁻ and hADSCs administration (a, c). The representative images of M2 macrophages (F4/80⁺ (red)/CD206⁺ (green)) after the PBS⁻ (e) and hADSCs (f) injection 7 days after surgery. Seven days after the hADSCs injection into ischemic muscle of immunodeficient NOD SCID mice, the infiltration of macrophages was not observed. The graph of the total area of F4/80 macrophages shows that there is no significant difference in the macrophages presence in the muscles between PBS⁻ and hADSCs groups (g). The number of capillaries (CD31⁺) in collected muscles 7 and 14 days after hADSCs and PBS⁻ administration (h). n = 5; 5 muscles per group were analyzed; 5 (for the total area of F4/80) or 10 (for the number of capillaries) pictures of each muscle were taken. *p < 0.05, ***p < 0.001 evaluated with U Mann-Whitney test. Nuclei stained with DAPI (blue), scale bars = 50 μm