Fig. 2

Hypoxia inhibited osteo/odontogenic differentiation in DPSCs. a ALP activity of DPSCs was reduced in the hypoxia group 5 days after osteo/odontogenic induction. b, c Alizarin red staining (b) and calcium quantitation (c) showed a significant decrease in mineralization of DPSCs in the hypoxia group 2 weeks after osteo/odontogenic induction. d–f Real-time RT-PCR indicated that the expression levels of BSP, OCN, and DSPP in DPSCs in hypoxic conditions were inhibited 7 and 14 days after osteo/odontogenic induction. g Western blot results showed that hypoxia inhibited the expressions of BSP, OCN, and DSPP at 2 weeks after osteo/odontogenic induction. GAPDH was used as an internal control for real-time RT-PCR, and β-actin was used as an internal control for western blot analysis. Student’s t tests were performed to determine statistical significance. All error bars represent SD (n = 3). *p < 0.05, **p < 0.01