Fig. 6

Knockdown of the lncRNA STL inhibited the osteo/odontogenic differentiation potential of DPSCs in vitro. a STL expression increased during osteo/odontogenic differentiation of DPSCs evaluated by real-time RT-PCR. b Real-time RT-PCR showed knockdown efficacy of STL. c ALP activity assay showed that STL depletion reduced ALP activity. d, e STL depletion repressed the mineralization of DPSCs shown by Alizarin red staining (d) and calcium quantitative analysis (e). f Western blot results showed that knockdown of STL inhibited the expressions of BSP and DSPP in DPSCs after osteo/odontogenic induction. g, h STL depletion inhibited NQO1 expression (g) but promoted ERO1L expression in DPSCs (h). GAPDH was used as an internal control for real-time RT-PCR, and β-actin was used as an internal control for western blot analysis. One-way ANOVA and Student’s t tests were performed to determine statistical significance. All error bars represent SD (n = 3). NS; no significant difference, *p < 0.05, **p < 0.01