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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Exploiting tumor-intrinsic signals to induce mesenchymal stem cell-mediated suicide gene therapy to fight malignant glioma

Fig. 4

hAMSC-SBE4-TRAIL increased apoptosis in human primary GBM in vitro. a Representative images showed the flow cytometry of annexin V and PI assay. b–d The GBM cells (GBM025, GBM079, and GBM106) cultured in SBE4-TRAIL-C-CM displayed a significant increasing in apoptosis than cultured in control-C-CM, vector-C-CM, and SBE4-TRAIL+TβR inh-C-CM conditions. eg Western blot assay cleaved caspase-8 and cleaved caspase-3 were detected in the SBE4-TRAIL group, but were essentially absent in vector and SBE4-TRAIL+TβR inh group. GBM025, GBM079, and GBM106 co-cultured with SBE4-TRAIL exhibited a higher level of cleaved caspase-8 as well as cleaved caspase-3 when compared with other groups. Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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