Table 1 Primers used to perform qRT-PCR
From: Impact of HOXB7 overexpression on human adipose-derived mesenchymal progenitors
Gene | Primer sequence | Amplified length (bp) |
|---|---|---|
β-actin | 5′-ACCTTCTACAATGAGCTGCG-3′ (sense) 5′-CCTGGATAGCAACGTACATGG-3′ (antisense) | 148 |
Ki67 | 5′-GTCGTGTCTCAAGATCTAGCTTC-3′ (sense) 5′-GTCATCTGCGGTACTGTCTTC-3′ (antisense) | 146 |
HOXB7 | 5′-CCTGGATGCGAAGCTCAG-3′ (sense) 5′-CGTCAGGTAGCGATTGTAGTG-3′ (antisense) | 107 |
bFGF | 5′-ACCCTCACATCAAGCTACAAC-3′ (sense) 5′-AAAAGAAACACTCATCCGTAA-3′ (antisense) | 141 |
ALP | 5′-GATGTGGAGTATGAGAGTGACG-3′ (sense) 5′-GGTCAAGGGTCA GGAGTTC-3′ (antisense) | 142 |
COL1A1 | 5′-CCCCTGGAAAGAATGGAGATG-3′ (sense) 5′-TCCAAACCACTGAAACCTCTG-3′ (antisense) | 148 |
DCN | 5′-AAAATGCCCAAAACTCTTCAGG-3′ (sense) 5′-GCCCCATTTTCAATTCCTGAG-3′ (antisense) | 146 |
PPAR-γ | 5′-GAGCCCAAGTTTGAGTTTGC-3′ (sense) 5′-GCAGGTTGTCTTGAATGTCTTC-3′ (antisense) | 148 |
LPL | 5′-GAAGACTCGTTCTCAGATGCC-3′ (sense) 5′-GAATGGGATGTTCTCACTCTCG-3′ (antisense) | 145 |
SOX9 | 5′-ACTTGCACAACGCCGAG-3′ (sense) 5′-CTGGTACTTGTAATCCGGGTG-3′ (antisense) | 140 |
COL2A1 | 5′-CCTCAAGGATTTCAAGGCAATC-3′ (sense) 5′-ACCCCTTTCACCAGCTTTTC-3′ (antisense) | 144 |
p21 | 5′-GAGGCCGGGATGAGTTGGGAGGAG-3′ (sense) 5′-CAGCCGGCGTTTGGAGTGGTAGAA-3′ (antisense) | 221 |
p53 | 5′-GAGCTGAATGAGGCCTTGGA-3′ (sense) 5′-CTGAGTCAGGCCCTTCTGTCTT-3′ (antisense) | 151 |