Fig. 4

Characterisation using flow cytometry on Quantum®- and TCP-expanded BM-MSCs and UC-MSCs at P1. No significant differences in immunoprofile were noted between culture conditions; however, BM-MSCs (a) expressed significantly more CD271 and CD34 compared to UC-MSCs (b) after P1 expansion in the Quantum® (p = 0.029). Cell surface marker immunopositivity following IFN-ϒ stimulation on P1 Quantum®- and TCP-expanded c BM-MSC and d UC-MSC re-seeded onto TCP at P2 was comparable for the co-stimulatory markers CD40, CD80 and CD86. Some BM-MSCs grown on TCP and UC-MSCs grown in the Quantum® at P1 and stimulated with IFN-γ at P2 were immunopositive for HLA-DR. All of the cultures tested upregulated CD106 and CD317 in response to IFN-γ, with no significant difference between the Quantum®- and TCP-expanded cells. Mean values are plotted ± standard deviations