Fig. 6

RT-qPCR analyses for the expression of immunomodulatory genes in Quantum®-expanded BM- and UC-MSCs. a BM-MSCs and b UC-MSCs cultured in the Quantum® bioreactor at passage 2 following stimulation with 25 ng/ml IFN-γ for 24 h. Only IDO was consistently and significantly upregulated in response to IFN-γ stimulation in both cell types. Gene expression was normalised to GAPDH and HPRT1. Gene expression for IFN-γ-stimulated cells is expressed relative to those grown in normal media without the inflammatory stimulus. Stars indicate genes which are significantly up- or downregulated. Mean values are plotted ± standard deviations