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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The non-homologous end-joining activity is required for Fanconi anemia fetal HSC maintenance

Fig. 3

Inhibition of NHEJ causes fetal HSC depletion in Fanca−/− embryos. a DNA-PKcs3A/3A induces fetal HSC depletion in Fanca−/− embryos. Fetal liver cells from E14.5 embryos with the indicated genotype were subjected to flow cytometric analysis for fetal HSC (CD150+CD48LinMac-1+Sca-1+). Representative flow cytometric plots (left) and quantification (right) are shown. ***p < 0.001 vs WT control. b Increased apoptosis in DNA-PKcs3A/3A Fanca−/− fetal HSCs. Fetal HSCs from E14.5 embryos of the indicated genotype were analyzed for apoptosis by Annexin V and 7AAD. Representative flow cytometric plots (left) and quantification (right) are shown. *p < 0.05 or ***p < 0.001 vs WT control. c Decreased quiescence in DNA-PKcs3A/3A Fanca−/− fetal HSCs. The percentage of quiescent (G0) fetal HSCs in E14.5 embryos of the indicated genotype. Representative flow cytometric plots (left) and quantification (right) are shown. *p < 0.05 or **p < 0.01 vs WT control

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