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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: A critical role for miR-184 in the fate determination of oligodendrocytes

Fig. 2

Ectopic expression of miR-184 induces OL specification in mouse cortex. a Mouse embryos were electroporated with pLenti-III-Ctrl or pLenti-III-miR-184 at E14.5 and harvested at E17.5. The sections of electroporated cortices were evaluated by immunostaining with antibodies against early and late OL markers, respectively. b Relative fluorescence intensity (G/B) representing the green fluorescence intensity (G) normalized to that of the blue one (B) was obtained for IHC results after analysis of the images by ImageJ software. Unpaired t-test method was employed in each case to compare the amounts of results statistically. c Expression of lineage-specific markers was measured on the electroporated cortices (n = 3) at a defined cortical area (1 mm2). β-Actin was used as an internal control. Electroporation of miR-184 induced an increase in the OL genes and a decrease in the astrocyte and neuron-specific markers in the cortex. Data represent mean ± SD. (*P < 0.05). d MBP protein level was also evaluated by western blotting in the electroporated cortices (n = 3) at a defined cortical area. GAPDH is used as the control in western blot analysis. e Average relative density of protein bands was obtained after densitometric analysis of the bands by ImageJ software followed by normalization to that of GAPDH as the internal loading control. Unpaired t-test method was employed to compare the amounts of results statistically. *P value < 0.05. f Myelination was examined using luxol fast blue-crystal violet staining on electroporated sections

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