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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A critical role for miR-184 in the fate determination of oligodendrocytes

Fig. 3

Downregulation of miR-184 by antimiR reduces the expression of OL lineage markers. a NPCs were transduced with pLenti-III-miR-Off-184, pLenti-III-Scr, and pLenti-III-Ctrl. mRNA levels of lineage-specific markers were quantified 7 days post-transduction. β-actin was used as an internal control. Columns, mean of three different experiments; bars, SD; (P < 0.01). b Histogram depicts the percentage of OLIG2+ and NKX2.2+ cells 7 days in oligo medium and MBP+ after 2 days in growth factors free oligo medium using ICC. Knockdown of miR-184 decreased the percentage of OLIG2+ and NKX2.2+ cells significantly compared to the control. Interestingly, no MBP+ cells were observed in the antimiR group. Data obtained from at least three independent experiments. In each ICC experiment, 100 cells were counted and the number of marker-positive cells was reported as a percentage. Data represent mean ± SD. (*P < 0.05)

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