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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes

Fig. 3

ASC-EVs are taken up by synoviocytes. a CFSE-EVs incorporated by FLSs after 24-h co-culture at different EV:FLS ratios. Number of incorporated EVs has been calculated comparing FLS fluorescence with signal given by a pre-determined number of isolated CFSE-labeled EVs (Quantification of data from three independent FLSs, each incubated with three independent ASC-EVs, is shown as mean ± SD. ****p < 0.0001; ***p < 0.001; **p < 0.01). b Flow cytometry of CFSE-EV-treated FLSs at 100,000:1 ratio showing that all cells incorporate fluorescent vesicles. c Confocal microscopy images of FLSs after 24-h co-culture with labeled ASC-EVs. Uptaken EVs localize in perinuclear areas. d Kinetics of EV incorporation (100,000 EVs per FLS, n = 3 independent experiments, each FLS incubated with a (1:1:1) mix of ASC-EVs from three independent ASCs. Data are presented as mean ± SD. *p ≤ 0.05). e FLSs, after 24-h incubation with CFSE-EVs, are able to release fluorescent vesicles (box). Representative plot of three independent analyses

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