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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes

Fig. 4

HA coat has a crucial role in EV incorporation. a Ratio of ASC-EV incorporation with (+) or without (−) HA coat, after 24 h [100,000 EVs per FLS, n = 3 independent experiments, each FLS incubated with a (1:1:1) mix of ASC-EVs from three independent ASCs]. Data are presented as mean ± SD. **p < 0.01, *p ≤ 0.05, ns > 0.05. b ASC-EVs release from FLSs after coat digestion with hyaluronidase (+HYAL) for 10 m at 37 °C. Digestion of FLSs with (+EV) or without (−EV) 24-h pre-incubation with ASC-EVs is presented. Released vesicles in the green box. c Ratio of ASC-EV incorporation with (+) or without (−) 2 mg/ml soluble high molecular weight HA in growth medium, after 24 h [100,000 EVs per FLS, n = 3 independent experiments, each FLS incubated with a mix (1:1:1) of ASC-EVs from three independent ASCs]. Data are presented as mean ± SD. **p < 0.01, *p ≤ 0.05, and ns > 0.05

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