Fig. 4

Adipocyte exosomes reduce breast cancer cell apoptosis. a Flow cytometric analysis of SS-induced apoptosis in MCF7 cells treated with or without adipocyte exosomes. Cells in the lower right quadrant (Annexin V+/PI−) are the early apoptotic cells, and those in the upper right quadrant (Annexin V+/PI+) represent late apoptotic cells. The experiments were performed in triplicate. The representative images are shown at the left, and the quantitative data are presented at the right. The data are presented as the mean ± SEM (n = 3). b Flow cytometric analysis of 5FU-induced apoptosis in MCF7 cells treated with or without adipocyte exosomes. The representative images are shown at the left, and the quantitative data are presented at the right. The data are presented as the mean ± SEM (n = 3). c Flow cytometric analysis of MCF7 cells cultured in an adipocyte-conditioned medium (without serum) and exosome-depleted adipocyte-conditioned medium (without serum) for 48 h. The representative images are shown at the left, and the quantitative data are presented at the right. The data are presented as the mean ± SEM (n = 3). d Flow cytometric analysis of 5FU-induced apoptosis in MCF7 cells treated with a culture medium or exosome-depleted culture medium. The representative images are shown at the left, and the quantitative data are presented at the right. The data are presented as the mean ± SEM (n = 3)