Fig. 7

Adipocyte exosomes activated the Hippo signaling pathway in MCF7 cells. a Phosphorylation of YAP, Jak2, JNK, P38, ERK, Stat3, and AKT was analyzed by western blot. GAPDH was used as the loading control. b–e Phosphorylation of key proteins was analyzed after addition of specific signaling pathway inhibitor. b WP1066 inhibitor of JAK2/STAT3. c SP600125 inhibitor of JNK. d Verteporfin inhibitor of Hippo. e U0126 inhibitor of ERK/P38. f MTS analysis of MCF7 cells treated with adipocyte exosomes or PBS in the presence or absence of different signaling pathway inhibitors. g Representative immunofluorescence staining images of nuclear translocation of TAZ in adipocyte exosome-treated MCF7. h Phosphorylation of key proteins of the Hippo signaling pathway. i Representative immunofluorescence staining images of nuclear translocation of YAP in adipocyte exosome-treated MCF7. j Expression of YAP/TAZ downstream genes. GAPDH was used as an internal control