Fig. 4

IL-33 protects HSCs from apoptosis after irradiation. a The percentage of annexin⁺ bone marrow Lin⁻c-Kit⁺ cells at 72 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. b The percentage of annexin⁺CD45⁺ cells in the bone marrow at day 7 after 600-cGy TBI and treatment with PBS or IL-33. Data are mean ± SEM (n = 6 in each group). *P < 0.05 compared to untreated controls. c Left, P53 mRNA expression in bone marrow Lin⁻c-Kit⁺ cells at 6 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Right, mean percentages of P53 protein expression in Lin⁻c-Kit⁺ cells at 36 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. d The percentage of annexin⁺ Lin⁻c-Kit⁺ cells with or without P53 inhibition at 72 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. e Left, PUMA mRNA expression in bone marrow Lin⁻c-Kit⁺ cells at 6 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Right, mean percentages of PUMA protein expression in Lin⁻c-Kit⁺ cells at 36 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. f The percentage of annexin⁺ Lin⁻c-Kit⁺ cells with or without PUMA knockdown at 72 h of culture with TSF or TSF plus IL-33 and after 200-cGy irradiation and the culture conditions are shown. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant. g CFCs from bone marrow Lin⁻c-Kit⁺ cells with or without PUMA knockdown in irradiated TSF culture and irradiated TSF plus IL-33 culture groups. Data are mean ± SEM (n = 3 in each group). *P < 0.05 compared to untreated controls. n.s. not significant