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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: IGF-1 overexpression improves mesenchymal stem cell survival and promotes neurological recovery after spinal cord injury

Fig. 1

Increased survival of BMMSC-IGF1 and induction of cell proliferation in the injured spinal cord. Presence of GFP+ BMMSCs was detected in spinal cord sections of mice transplanted with wild-type BMMSCs (a) or BMMSC-IGF1 (b), visualized in green by confocal microscopy, 5 days after spinal cord injury and cell transplantation. Nuclei were stained with DAPI (blue). Scale bars = 50 μm. c Quantification of GFP mRNA in the injured spinal cord segments, isolated at 5 days post-injury and transplant, measured by qRT-PCR. Confocal microscopy of injured mouse spinal cords, 5 days post-injury and transplanted with wild-type BMMSC (d) or BMMSC-IGF1 (e), immunostained for the proliferation marker Ki-67 (red) and nuclei stained with DAPI (blue). Scale bars = 200 μm. f Quantification of Casp3+-labeled cells in spinal cord sections. Values represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001

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