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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: OCT4 and PAX6 determine the dual function of SOX2 in human ESCs as a key pluripotent or neural factor

Fig. 4

SOX2 interacts with OCT4 and PAX6 in hESCs and hNECs. a Dynamic mRNA expression of PAX6 and BRN2 by qRT-PCR during the neural differentiation of H1 hESCs as shown in Additional file 1: Figure S1. b Immunoblot of PAX6 and BRN2 protein expression in cells of a. c Immunostaining with indicated antibodies during the neural differentiation of hESCs. Days of differentiation are indicated. Scale bar = 50 μm. d qRT-PCR analysis of H1 hESCs 48 h after ectopic PAX6 expression. Data are presented as mean ± SD from three transduction experiments. **p < 0.01, ***p < 0.0005. e Immunoblot showing the effect of PAX6 overexpression on OCT4 in hESCs with quantification shown below. f Co-IP showing that SOX2 interacts with OCT4 in undifferentiated hESCs. g Co-IP showing that SOX2 interacts with PAX6 in hESC-derived neural epithelial cells

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