Fig. 6

Immunohistochemical analysis of α-SMA expression and desmin in wound sections. For quantitative assessment of myofibroblast proliferation, the number of positive cells for α-SMA (a) or desmin (b) was determined in ten random sections at × 400 magnification from three different mice of each group. Results are shown as mean ± SD. Myofibroblast proliferation was significantly increased compared to the untreated group (Linitul) (***p < 0.001) or significantly decreased compared to the Wj-MSCs-Edge-treated group (^###p < 0.001), respectively, according to one-way ANOVA. Experimental groups: SF (wounds covered with silk fibroin scaffold), Wj-MSCs-Edge (Wj-MSCs injected at the wound edge), Wj-MSCs-SF (wounds covered with silk fibroin scaffold cellularized with Wj-MSCs), and Wj-MSCs-SF+Edge (wounds treated with Wj-MSCs injected at the wound edge and cellularized silk fibroin scaffold onto the wound bed)