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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Hypoxia promotes differentiation of adipose-derived stem cells into endothelial cells through demethylation of ephrinB2

Fig. 1

hASCs were induced with a combination of VEGF (50 ng/ml) and BMP4 (100 ng/ml) under either normoxia or hypoxia circumstances for 14 days. A Flow cytometry analysis of CD markers in hASCs. B Osteogenic, adipogenic, and chondrogenic differentiation of hASCs was determined by ALP and Alizarin red staining, oil red staining, and immunofluorescent staining for collagen type II, respectively. C Morphology changes of hASCs under phase-contrast microscope observation. Scale bars: 50 um. Human umbilical vein endothelial cells (HUVECs) served as a positive control. D Proliferation of ASCs under different conditions determined by DNA assay using Hoechst 33258 dye

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