Fig. 3

Functional characteristic of vascular ECs differentiated from hASCs after treatment with VEGF (50 ng/ml) and BMP4 (100 ng/ml) for 14 days under hypoxia. A Tube formation (a–f) of induced hASCs on Matrigel and quantitative calculation of tube area (g), total loops number (h), mean loop area (i). Scale bar: 50 um. B Uptake of Dil-Ac-LDL by induced hASCs under indicated conditions. Scale bar: 20 um. C Nitric oxide production of induced hASCs. HUVECs served as a positive control. Data are presented as mean ± SD. *P < 0.05