Fig. 4From: Hypoxia promotes differentiation of adipose-derived stem cells into endothelial cells through demethylation of ephrinB2Detection of blood flow perfusion in the hindlimb of diabetic mice subjected to femoral artery ligation as well as hASC transplantation after 14 days. A Representative gross view of the ischemic hindlimb received transplantation of hASCs. B Quantitative analysis of amputation, foot necrosis, and limb salvage of mice in each group (n = 6). Blank control: normal hindlimb without intervention. C Representative images of blood flow perfusion detected by full-field laser perfusion imager. D Quantification of blood flow recovery presented as the ratio of ischemic to non-ischemic limb (from the knee to toe) in blood blow. E Immunofluorescent staining of CD31-positive cells in the ischemic subcutaneous tissue. Scar bar = 20 um. F Quantification of percentage of CD31-positive cells identified by immunofluorescent staining. Data are presented as mean ± SD. *P < 0.05. G Co-localization of CM-DIL-labeled hASCs and endothelial cells immunostained with anti-CD31 antibody. Arrowheads indicate co-localization of label hASCs with CD31-positive endothelial cells. Scar bar = 20 umBack to article page