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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Hypoxia promotes differentiation of adipose-derived stem cells into endothelial cells through demethylation of ephrinB2

Fig. 4

Detection of blood flow perfusion in the hindlimb of diabetic mice subjected to femoral artery ligation as well as hASC transplantation after 14 days. A Representative gross view of the ischemic hindlimb received transplantation of hASCs. B Quantitative analysis of amputation, foot necrosis, and limb salvage of mice in each group (n = 6). Blank control: normal hindlimb without intervention. C Representative images of blood flow perfusion detected by full-field laser perfusion imager. D Quantification of blood flow recovery presented as the ratio of ischemic to non-ischemic limb (from the knee to toe) in blood blow. E Immunofluorescent staining of CD31-positive cells in the ischemic subcutaneous tissue. Scar bar = 20 um. F Quantification of percentage of CD31-positive cells identified by immunofluorescent staining. Data are presented as mean ± SD. *P < 0.05. G Co-localization of CM-DIL-labeled hASCs and endothelial cells immunostained with anti-CD31 antibody. Arrowheads indicate co-localization of label hASCs with CD31-positive endothelial cells. Scar bar = 20 um

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